基于RNA-Seq识别山羊肉品质候选基因

doi:10.11843/j.issn.0366-6964.2015.08.004

Abstract

Abstract:

To screen candidate genes which effect on meat quality of cashmere goats，we explored differentially expressed genes of longissimus dorsi in cashmere goats with different ages and genders.The high-throughput sequencing for transcriptome of longissimus dorsi in cashmere goats was performed by RNA-Seq.Differentially expressed genes were selected as candidates genes and enriched based on GO and KEGG database after quality control of RNA-Seq.The 263 candidate genes including 123 beneficial and 140 deleterious genes were selected by CLC Genomics Workbench 6.0 software.The results of GO enrichment showed that the highly expressed beneficial genes were primarily related to growth and development of skeletal muscle，organelle formation and protein binding.The highly expressed deleterious genes were primarily related to lipid metabolic process，cytoskeleton and binding.According to the KEGG database，these genes were mainly involved in glycolysis/gluconeogenesis’mitogen-activated protein kinase，complement and coagulation cascades and tryptophan metabolism.As example，ADIPOQ，PDK4 and CD36 might be important candidate genes involved in meat quality，when combining with other livestock genomics study.These results provided theoretical basis for improving meat quality and studying candidate genes in cashmere goats.

CLC Number:

S827.2

MENG Xian-ran，DU Chen，WANG Jing，FU Shao-yin，ZHENG Zhu-qing，ZHANG Wen-guang，LI Jin-quan. RNA-Seq Approach for Identifying Candidate Genes of Meat Quality in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1300-1307.

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RNA-Seq Approach for Identifying Candidate Genes of Meat Quality in Goats

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